Proteins bound to the beads were eluted by denaturing elution buffer, separated by SDS-PAGE and immunoblotted with antibodies against proapoptotic and antiapoptotic proteins to identify the binding of Bcl-2 family proteins.
Bound proteins were eluted with SDS-sample buffer, separated by SDS-PAGE and analysed by autoradiography.
Bound proteins were eluted with 2× SDS buffer, separated by SDS-PAGE and visualised by autoradiography.
Finally, proteins were eluted from the beads by 2XSDS buffer separated by 12% SDS-PAGE, and visualized by autoradiogram.
The bound proteins were eluted with SDS sample buffer, separated by SDS-PAGE, and visualized by autoradiography.
Bound proteins were eluted by boiling in 1× SDS sample buffer, separated by SDS-PAGE.
Bound proteins were eluted with SDS sample buffer, separated by SDS-PAGE and analysed by immunoblotting.
Samples were harvested in RIPA buffer, separated by SDS-PAGE and electrotransferred onto nitrocellulose membranes.
Immunoprecipitates were washed five times with lysis buffer, separated by SDS-PAGE, and transferred to membranes.
After incubation, bound proteins were eluted by SDS sample buffer, separated by SDS-PAGE and visualized by Coomassie Blue staining.
