Sentence examples for binding buffer for at from inspiring English sources

All these fractions were combined, washed by PBS and incubated with PE- or Dy647-labeled annexin V (Becton Dickinson and Apronex, Vestec, Czech Republic) in the supplied binding buffer for at least 10 min.

For Figure 5B, recombinant His6-tagged Chordin and His6-tagged CV2-T5P/I21R or His6-tagged CV2 ΔClip were mixed and incubated in 200 µl binding buffer for 1h at 4°C, followed by BMP2.

HX1, GFP-HX1 and 88GFP-HX1 cells at 48 hpi with SGIV (MOI of 0.1) were trypsinized into single cell suspension and 105 cells were stained with 5 μL of Annexin V/pacific blue (Invitrogen, USA) in 100 μL of binding buffer for 15 min at room temperature and counterstained with PI at 50 μg/mL.

For each 25 µl reaction, 10 µg nuclear extracts were incubated with 32P-labeled probes in binding buffer for 20 minutes at room temperature.

Two µg of nuclear extracts and 20 fmol biotin-labeled DNA probe were incubated in 1x binding buffer for 30 min at room temperature.

EBV-transformed B cells (3×105 per sample) were preincubated in the presence of varying concentrations of TP5 in binding buffer for 2 h at 37°C.

Bound toxin was detected by incubation with rabbit antibodies against Delta or mouse monoclonal antibody against Beta toxin in binding buffer for 1 hr at room temperature followed by three washes with binding buffer.

Briefly, 20 µg nuclear proteins were pre-incubated with binding buffer for 10 min at 4°C and then incubated with biotin-labeled AP1 probe for another 20 min at room temperature.

Wells were then incubated with protein A-horseradish peroxidase (Bio-Rad) 1∶3000 or anti-mouse immunoglobulin peroxidase conjugate (Amersham-biotechs) in binding buffer for 1 hr at room temperature.

Briefly, 1×106 cells were washed twice with PBS, and stained with 5 µl of Annexin V-FITC and 10 µl of PI (5 µg/ml) in 1 ml binding buffer for 15 min at room temperature in the dark.

FLNa or its truncates were incubated with 30 µl of amylose resin (50% vol/vol slurry, New England Biolabs) coated with various concentration of MBP-FilGAP 649–748 in the binding buffer for 1 h at 25°C.