Sentence examples for binding buffer followed by from inspiring English sources

Before each experiment, biosensors were pre-equilibrated with binding buffer, followed by equilibrium binding with Sdo1p variants (2 μg/mL).

The cells were trypsinized, collected, washed, and finally suspended in one-time binding buffer, followed by staining with an Annexin-V antibody labeled with Alexa Fluor-488 for 15 min at RT in the dark.

Briefly, 105 resuspended cells were gently resuspended in 195 μL of Annexin V-FITC binding buffer followed by the addition of 5 μL of Annexin V-FITC and incubation in the dark at room temperature (20°C 25°C) for 10 min.

The chip was washed 3 times (5 min each) with binding buffer followed by a brief rinse with water.

Briefly, cells were incubated with Annexin V-FITC in Annexin binding buffer followed by staining with 5 µg/ml PI and analysis by flow cytometry.

Collected cells were washed with serum-containing media before being resuspended in 500 µl of 1× binding buffer, followed by addition of 5 µl of Annexin V-FITC and 5 µl of PI to the cell suspension.

Chromatin was isolated by the addition of lysis buffer, followed by disruption with a Dounce homogenizer.

The highest ratio of difference was presented by M13KO7 blocked in PTM buffer, followed by a combination of PTM buffer and lysate and lysate only.

The packed cells were washed with isotonic buffer, followed by lysis with hypotonic buffer.

Slides were then washed in phosphate buffer followed by three Tris-base buffer washes.

Application of cell lysate in binding buffer was followed by a washing step with 10 ml of binding buffer containing 60 mM imidazole and an elution step with 5 ml of elution buffer (50 mM Hepes/NaOH, pH 8.0, 300 mM NaCl and 500 mM imidazole).