Sentence examples for binding buffer composed of from inspiring English sources

HEK-293 EBNA cells expressing MC receptors were harvested from plate and resuspended in binding buffer composed of 25 mM HEPES, 2.5 mM CaCl2, 1 mM MgCl2 and 0.2% bacitracin, pH adjusted to 7.4.

and incubated with 200 μl of binding buffer composed of (all in mM) 25 Tris HCl (pH 7.5), 1 DTT, 30 MgCl2, 40 NaCl, and with added 0.5 % Nonidet P-40, and 5 μl glutathione-Sepharose 4B beads at 4 °C.

The pooled binding fractions were further concentrated and the buffer exchanged to a buffer composed of 250 mM 6-aminocaproic acid, 25 mM Bis Tris, pH 7.0 using Amicon ultra centrifugal filter devices.

For the bR synthesized in the PURE system, an assay buffer composed of 10 mM HEPES-KOH (pH 7.5), 5 mM MgCl2 and 100 mM KCl was used.

The placebo consisted of the viral formulation buffer, composed of sodium chloride, Tris buffer, trehalose (low endotoxin), magnesium chloride, monooleate (Tween 80) and water for injection.

The third scenario (randomized buffer) was a map of the core area plus a 25-km wide buffer composed of randomized habitat data (Figure 3c).

We recommend the use of a buffer composed of randomized habitat data as a solution to this problem.

Cells were suspended in buffer composed of PBS containing 1% FCS.

The DNA binding reaction mixture contained ∼100 000 c.p.m. of the P-end-labelled double-stranded oligonucleotide (∼1 ng), plus ∼5  μg of crude protein extract, 1  μg of poly (dI-dC -poly (dI-dC -polyma) andI-dC -polymposed of 10 m M HEPES (pH 7.9), 50 m M NaCl, 1 m M DTT, 1 m M EDTA, and 5% glycerol.

The wells were then treated with a blocking buffer composed of 5% BSA in PBS and washed with buffer composed of 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, and 0.1% Tween 20.

DVCs was generally lower in landscapes (100- and 1000 m buffers) composed of land that is more open.