Sentence examples for binding buffer and then from inspiring English sources

Dissociated cells were washed with phosphate-buffered saline (PBS) two times, resuspended in 500  μL binding buffer, and then incubated for 1 hour at room temperature in the presence of 0.5  μg/mL annexin V-FITC (R&D) and 5  μL propidium iodide for 5 minutes in bindingbuffer as described by the manufacturer.

The pellet was then re-suspended in 200 μl binding buffer and then centrifuged again at 1200 rpm for 5 min and the supernatant removed.

Cells were resuspended in 195 µL of binding buffer, and then incubated with annexin V-FITC and PI for 15 min in the dark at room temperature.

All suspensions were washed three times with PBS, suspended in 500 μl of binding buffer, and then analyzed by flow cytometry (Epics XL, Beckman Coulter, USA) using FLOWJO 7.6 software.

Cells were rinsed with 1× binding buffer and then incubated with 200 μl of 1× binding buffer containing 5 μl of the annexin V solution and 10 μl of the PI solution; then, 5 μg/ml of Hoechst 33342 (H1399 from Invitrogen) was used for nucleus staining (blue).

The protein-bound resin was loaded onto a column, washed with 20 ml of binding buffer and then washed sequentially using the binding buffer containing 20 and 40 mM imidazole.

Agarose bead-DNA-protein complexes were washed three times with ice cold binding buffers and then were eluted in SDS-PAGE loading buffer by heating at 95°C.

Tritiated colchicine (100  μl; Perkin-Elmer; specific activity 70 80 Ci mmol−1) was added to 300  μl of tubulin-binding buffer and then 10  μl was added in each well.

Briefly, the lysate was cleared by centrifugation and then incubated with streptavidin sepharose for 2 hours at 4°C under constant rotation, washed three times with the provided streptavidin binding buffer and was then eluted with streptavidin elution buffer.

After removing the unbound I-echistatin, the hydrophilic PVDF filters were washed three times with the binding buffer, and were then collected.

The column was washed with binding buffer, and proteins were then eluted with 10%, 60%, and 100% elution buffer, containing 20 mM potassium phosphate buffer, 500 mM NaCl, and 500 mM imidazole (pH 7.4).