Sentence examples for binding buffer and subsequently from inspiring English sources

The cells were washed twice with binding buffer and subsequently incubated at 37°C for 10 min in 1.0 ml binding buffer.

The column was washed with 10 ml of binding buffer and subsequently with 15 ml of washing buffer (20 mM Tris HCl buffer containing 500 mM NaCl and 60 mM imidazole, pH 7.9).

After incubation, the beads were washed five times with the binding buffer and subsequently boiled and separated on a 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel.

Cells were resuspended in 400 μl Annexin V binding buffer and subsequently incubated with 5 μl Annexin V-fluorescein isothiocyanate for 15 minutes in room temperature; then, 10 μl of propidium iodide was added.

The trypsin solution was removed and a small piece of the seminiferous tubules was minced in annexin-V binding buffer and subsequently incubated with annexin V (Annexin V-FITC Apoptosis Kit, BioVision, Milpitas, CA, USA) at room temperature for 5 min in the dark.

The cleared lysate was loaded onto a column pre-packed with 10 g DE52 (Whatman) anion exchange resin (previously activated with 2.5 M NaCl and equilibrated with binding buffer), and subsequently onto a 3-mL Ni-NTA (Qiagen) column pre-equilibrated with binding buffer.

The cells were washed with ice-cold phosphate-buffered saline (PBS; Sigma; cat. no. D8537), resuspended in Annexin V-binding buffer, and subsequently stained with AnnexinV-fluorescein isothiocyanate and PI (BD Pharmingen, San Jose, CA, USA), according to the manufacturer's recommendation (Società Chimici Italiani; cat. no. IK-11120).

Cells were subsequently resuspended in 1X Annexin V binding buffer and incubated with Annexin V-FITC (Pharmingen Biosciences, San Diego, CA, USA) for 15 minutes at room temperature.

Subsequently, after washing, cells were re-suspended in fresh binding buffer and stained with PI.

The cells were subsequently washed 2 times in annexin V binding buffer and then visualized on a Nikon inverted fluorescent microscope equipped with a digital camera.

After binding, the membrane was washed thrice with binding buffer and exposed for autoradiography.