Sentence examples for binding buffer and further from inspiring English sources

Then the microtiter plate was washed for three times with binding buffer and further incubated with 200  μL of Dig-labeled A10-2 (40 ng/ μL).

After treatment, cells were washed twice with warm PBS, detached by trypsin without EDTA, collected, centrifuged, washed with warm PBS, resuspended in the binding buffer and further stained with PI and Annexin V-FITC for 15 min at ambient temperature in the dark.

Murine lungs harvested from infected mice were homogenized in the Ambion Mirvana lysis-binding buffer and further extracted using the PCT pressure cycler (Pressure Biosciences).

Protein G beads (Sigma) were then washed with the binding buffer and incubated with the extracts for a further 2 h at 4 °C.

This was further supported by an experiment in which the binding buffer and gradient contained a high concentration of salt (0.5 M NaCl, Figure 2C).

Additional melting curve profiles for the buffer screens (binding buffer and elution buffer screens) are given in Supplementary Figures S1, S2 and S3.

The beads were washed twice in binding buffer and subsequently peptides were eluted using elution buffer (10% NH4OH, 25% acetonitrile in H2O).

For apoptosis analysis, cells were resuspended with binding buffer and stained with Annexin V and 7-AAD for 15 min at 25 °C in the dark.

In all, 50 μl of serum was added to 150 μl of binding buffer and mixed by vortex and loaded on the spin columns.

The columns were washed with 200 μl of the binding buffer and combined with the first eluate to form the depleted serum sample.

The kit contains annexin V-FITC (20 μg/ml in Tris-NaCl), 1× binding buffer and propidium iodide (PI; 50 μg/ml in 1× binding buffer).