Sentence examples for binding buffer and finally from inspiring English sources

Then cells were washed with PBS, resuspended in Annexin-V binding buffer and finally stained with Annexin-V-FITC and PI.

The cells were harvested and resuspended in binding buffer and finally 5  μL of Annexin V-FITC and 5  μL of 7-AAD were added.

Finally, cells were suspended in 1× binding buffer and analyzed within 1 h by FACS Calibur Flow Cytometer (Becton Dickinson, San Jose, CA, USA).

Finally, cells were washed three times with binding buffer and mounted in 70% glycerol for microscopy analysis.

Finally, cell samples were diluted with 400 μl 1× binding buffer and analyzed in a flow cytometer (FACSCalibur, Becton Dickinson, Franklin Lakes, NJ, USA) within 1 hour.

Finally the cells were added to 200 ul of 1× binding buffer and assayed immediately using FACSCanto flow cytometry (BD Biosciences, San Jose, CA).

The kit contains annexin V-FITC (20 μg/ml in Tris-NaCl), 1× binding buffer and propidium iodide (PI; 50 μg/ml in 1× binding buffer).

Cells were then washed three times with binding buffer and lysed in 1 M NaOH.

The beads were then washed 2 times with binding buffer and resuspended in 50 µl binding buffer.

The beads were washed for 3 times with the binding buffer and bound materials were eluated by incubating the beads with binding buffer comprising 4 mM biotin.

Cell pellets were suspended in 200 μl of binding buffer, and then incubated with 10 μl of Annexin V-FITC and 5 μl of Propidium Iodide at room temperature for 15 min. Finally, 300 μl of binding buffer was added into the incubation solution and was analyzed by flow cytometry (Becton Dicklnson, USA) in 1 h.