Sentence examples for binding blots were incubated from inspiring English sources

After washing blots to remove excessive primary antibody binding, blots were incubated for 1 hr with horseradish peroxidase (HRP conjugated secondary antibody (1∶5,000).

After three washes with TBS-T to remove excessive primary antibody binding, blots were incubated with horseradish peroxidase (HRP conjugated secondary antibody (1∶5,000) for 1 hr at room temperature.

To decrease nonspecific binding, blots were incubated with blocking solution containing 5% nonfat dry milk (Bio-Rad, Hercules, CA, USA) in Tris-buffered saline (TBS) with 0.05% Tween 20 (TBS/Tween) (Bio-Rad) for 1 hour at room temperature.

After 1-h blocking for nonspecific binding, blots were incubated with specific primary antibodies (1 10000 dilution) against α‐actin (GTX100095, GeneTex), α‐tubulin (sc-8035, Santa Cruz Biotechnology, Santa Cruz, CA, USA), PCNA (sc-56, Santa Cruz), NFκB (p65) (MAB3026, Santa Cruz), gp91 phox (07 024, Millipore) or β-catenin (06 734, Millipore).

After the primary antibody binding reaction, the blots were incubated with either anti-mouse or anti-rabbit horseradish peroxidase-conjugated secondary antibody (Amersham Biosciences, Little Chalfont, Buckinghamshire, UK) and developed using the enhanced chemiluminiscence detection system (Amersham) according to manufacturer's instructions.

After blocking for non-specific antibody binding (5% skim milk, TBS/0.1% tween-20 [TBS-T]), blots were incubated at 4°C overnight with diluted (5% skim milk, TBS-T) antibodies against FLAG® (polyclonal) (1∶500; Sigma) and GAPDH (1∶20,000; Abcam).

Blots were incubated with C4F6 overnight at 4 °C, and C4F6 binding was visualized using HRP-conjugated antimouse antibodies (GE Healthcare, Pierce, Millipore).

Nonspecific binding sites were blocked according to the recommendations of the manufacturer of each antibody and the blots were incubated with primary antibodies over night at 4°C.

The blots were incubated overnight in Tris buffered saline (TBS) containing 5% milk to block nonspecific binding of the antibody.

After blocking non-specific binding sites with 5% nonfat dry milk in Tris-buffered saline (TBS: 25 mM Tris-HCl, pH 7.4, 150 mM NaCl), blots were incubated with primary antibody overnight at 4°C.

Blots were incubated with primary antibodies overnight at 4 °C.