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RIP-Seq of Drosophila UNR in male and female flies identified a pronounced sex-specific binding bias for this protein; follow-up RNA-Seq experiments correlated UNR binding with the sex-specific alternative processing of UTRs in target messages [ 51].
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For PCR-based methods, longer sequences and multiplex reactions can be developed to avoid primer binding bias.
The third protocol deployed (IT8) was developed to identify binding peptides capable of binding directly to matrices, avoiding bias for the selection of peptide sequestration while retaining in situ biopanning on matrix sections.
In all populations tested, there was a λ chain bias for HIV Env binding antibodies, compared with other HIV antigens (such as p24) or tetanus toxoid.
A DnaK binding preference for L and negative bias for acidic residues is reported (18).
Furthermore, for the groupwise method, the resultant binding potential estimates had reduced variation and bias for individual scans and improved half maximal effective concentration (EC50) estimates were obtained for the study as a whole.
In yeast, there is a strong bias for promoters and transcription factor binding sites (TFBS) to be found in locations that have low intrinsic nucleosome occupancy [1].
Even a 50% difference in cerebellar specific binding between subjects would lead to only 3.5% bias for group comparisons in target regions (52).
As in WT cells, an initiation bias for ARS1 S was observed for pN&S indicating that neither Cst6 nor Yap3 binding is responsible for the bias.
Often small data sets contain a strong bias for both the negative and positive data since the data was selected to fit some predicted binding motif.
A bias for practical research.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com