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In this work, a systematic investigation was performed on the binding behaviour of the model drug dexamethasone with βCD derivatives functionalized with a small, negatively charged moiety (carboxyl groups, CM) and a large, neutral, reactive moiety (hydrazide groups, Hzd), both free and grafted to dextran.
Therefore, the binding behaviour of the synthesized peptides to HSA and BSA was carried out using the unique and intrinsic fluorescence from the tryptophan residues.
The binding behaviour of the super-agonists suggests that additional interactions are formed between the ligand and the IL-4 receptor α-chain.
Compared with RRASWT, aberrant binding behaviour of the two RRAS mutants was demonstrated, with RRASG39dup exhibiting an increased binding affinity towards PIK3CA, RAF1, PLCE1 and RASSF5, and RRASV55M to RALGDS.
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The grand canonical Monte Carlo (GCMC) method [37] is appropriate to describe the re-binding behaviour of the EMIC under study.
This is confirmed by the poor metal-binding behaviour of the same isoforms confronted with their non-cognate metal ions and by the inertness of the two complexes, Cd6-HpCdMT and Cu12-HpCuMT, to exchange metal ions.
In our experiments, non-treated and organophilised bentonites were tested to get an idea of the endotoxin binding behaviour of sorbents in different media, and whether the LAL assay can be used to quantify endotoxin activity in different media.
While this prediction often matches experimental results reasonably well, other factors besides the net charge can influence the binding behaviour of proteins to ion exchange columns.
Thus, we conclude that the drug binding pocket remains unchanged in the new strain and, hence, the binding behaviour of neuraminidase inhibitors such as oseltamivir (Tamiflu®) and zanamivir (Relenza®) should be unaffected.
The chloride-dependent spectral shift indicates the binding affinity of chloride ions to HRs, which is analogous to the binding behaviour of a ligand to a receptor, and it can be described via the Hill equation [ 21].
For the other four candidates of our proteomic screen, the binding behaviour of NCU00164, NCU05750 and NCU11333 remains to be established, while NCU03830 likely associates via protein-protein interactions.
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