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While this prediction often matches experimental results reasonably well, other factors besides the net charge can influence the binding behaviour of proteins to ion exchange columns.
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Hydrophobicity (and hydrophilicity) had been shown to have a major role in the binding behaviour of specific drugs and proteins [ 25].
Xiao et al. [ 53] examined the binding behaviour of FKBP12.6 with unphosphorylated and phosphorylated RyR2 proteins and did not find significant differences.
For the other four candidates of our proteomic screen, the binding behaviour of NCU00164, NCU05750 and NCU11333 remains to be established, while NCU03830 likely associates via protein-protein interactions.
We conclude from our data that the binding behaviour of P. falciparum iRBCs is highly promiscuous.
Furthermore, the binding of drugs to protein in vitro is considered as a model in protein chemistry to study the binding behavior of protein.
The slow-binding behaviour of DLGG (compound 11) indicates that DLGG might bind covalently to PPARγ.
Detailed information about the dynamic behaviour of proteins is essential for a proper understanding of a variety of processes, including catalysis, ligand binding and protein protein interactions, and could also prove useful in drug design.
It could be suspected that a remaining proteolytic activity in the stored protein suspension led to a partial digestion of some proteins, which might influence their binding behaviour, thereby simplifying separation.
Its binding behaviour was somewhat in between that of the wild-type and pCMF containing proteins and was different from RAD52Y104D mutant (Supplementary Figure S10B and C).
This, together with the fact that dengue viral proteins do have serotype-specific variations of structure and function, lead to the study of the serotype-specificity of the dengue β-OG ligand binding behaviour.
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