The results indicate that the datA locus might indeed prevent further initiations in a given cell cycle, since it titrates large numbers of DnaA-ATP molecules, effectively preventing them from binding at the origin (see Additional File 1, Figure A6A).
It is not clear the extent to which the relatively higher level of Pol1 binding at the origins at time zero reflects initial loading of Pol1, which may later be destabilized because of the failure of an increased loading of Pri1-Pri2.
Telomere repeat factor 2 (TRF2) binding at the EBV origin of plasmid replication (OriP) represses EBV replication.
Deletion of the Rpd3 histone deacetylase in S.cerevisiae leads to earlier Cdc45 binding at late origins and concomitant advancement in time of activation, resulting in a more rapid S-phase progression [2], [4].
In addition, we detected reproducible Mcm1 binding at two origin-recognition-complex components (ORC1 and 3) and the loading factor for the Mcm complex, CDC6 (Table 2; see Additional file 1).
On the other hand, we predict that mutations in the PLP-binding motif affect the interaction between PFN1 and PLP and many other ligands binding at this site15,16,17.
Like other TFs, OmpR may prevent binding of other repressors or directly activate transcription by recruiting RNAP, or exclude RNAP binding at this region to repress transcription.
By contrast, in Xenopus extracts a 90% reduction of MCM binding at origins still permits efficient in vitro replication [21].
Cdc6-E224Q also promoted MCM binding at origins in vivo but cells remained blocked in G1-phase.
These features probably contribute to origin specification in at least two ways: first, S. pombe ORC shows a strong preference for binding AT-rich DNA, and second, AT-richness may help create nucleosome-free regions which promote origin activity [84].
