Thus, the accumulation of huDysGFP in the fibre cytoplasm does not appear to affect the binding at the tips.
Thus, while cycles of ATP binding and hydrolysis can persist within the ring, the microtubule-binding domain at the tip of the stalk retains a strong-binding conformation.
Two-way coupling between the hydrolysis cycle in AAA1 and changes in MT-binding affinity at the tip of the stalk requires transmission both across the ring and along the stalk, a distance of ∼25 nm.
PilC is the extracellular 110 kDa adhesin [48] suggested as binding DNA at the tips of N. gonorrhoeae's type IV pili [49], [50].
In order for dynein to move along microtubules, ATP binding/hydrolysis at AAA1 must be coupled with linker motion and microtubule binding and release at the tip of the stalk, located 250 Å away (Gibbons et al., 2005; Imamula et al., 2007; Kon et al., 2009; Redwine et al., 2012).
Escherichia coli strains adhere to the normally sterile human uroepithelium using type 1 pili, that are long, hairy surface organelles exposing a mannose-binding FimH adhesin at the tip.
The full length Rep proteins contain a unique N-terminal DNA binding region which specifically recognizes the ITR at the 16 nt Rep binding element (RBE) and at the tip of one of the hairpin stems known as the RBE' (Fig. 1A) [4], [4].
The ATP-sensitive microtubule binding site is located at the tip of a 10 nm coiled coil stalk that emanates from between AAA4 and AAA5.
The first interaction of a phage particle and a bacterium is mediated through the specific recognition between the phage receptor binding protein (RBP), located at the tip of the tail, and the host cell receptor distributed over the cell surface.
