Sentence examples for binding at the secondary from inspiring English sources

It was suggested that transposase binding at the secondary site may down-regulate transposition.

The plate was incubated 1 hour at 37°C to allow the binding of the secondary antibody.

We defined our seed region as the first ten nucleotides from the 5' end of the miRNA to the 3' end of the miRNA and integrated various features including the number of consecutive matching bases in the seed region of 10 bases, a triplet feature in seed regions, thermodynamic energy, penalty of bulges and wobbles at binding sites, and the secondary structure of viral RNA for the prediction.

This is not a result of oligonucleotide binding at a secondary site as the same signals are observed regardless of input oligonucleotides and this pattern disappeared when a different batch of RNA is used as shown in blue.

Any resulting immunofluorescence would suggest unspecific binding of the secondary antibodies.

This may lead to a different structure of the 30S subunit since binding of primary binding proteins affects binding of the secondary and tertiary binding proteins [116].

Non-specific binding of the secondary antibody was prevented by incubation of the sections with 20% rabbit's serum in TBS for 1 h at 4 °C.

We conclude that oleic acid binding alters the secondary structure of MSI1, but this transition does not appear to induce aggregation at concentrations below the CMC.

A negative control blot was incubated with the secondary antibody alone, to check for nonspecific binding of the secondary antibody.

Cells stained with secondary antibody alone were used to compensate for intrinsic fluorescence and nonspecific binding of the secondary antibody.

Subtraction of background secondary antibody MFI from scFv binding resulted in negative numbers due to binding of the secondary antibody to the scFv and not the cell lines.