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In the mouse rpS16 gene, GABP binding at the initiator decreases transcription activity both in vivo and in vitro [ 12].
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Translation initiation begins with binding of the initiator Met-tRNAi to the 40S ribosomal subunit.
In prokaryotes, binding of the initiator proteins to origins itself provokes unwinding of origin DNA.
Although protein binding is the initiator of toxicity and cellular injury, secondary processes amplify and propagate it.
These include binding sites for the initiator protein, DnaA, and for the repressor of several ribonucleotide reductase genes, NrdR [7], [9].
In L. hongkongensis, there is an oriC containing eight 9-bp repeat elements known as DnaA boxes, which are potential binding sites for the initiator protein DnaA.
For comparison, HRP binding to the initiator-modified particles (Si@initiator) was also tested following the same procedure.
You will feel tremendous guilt at being the initiator of "the beginning of the end".
At 70% of ORC1/CDC6 sites within a DGC and proximal (within 10 Kbp) to H4K10Ac, the initiator binding site preceded the modified histone (examples can be seen at 0.35 and 1.25 Mbp in chromosome 7; Figure 1).
The binding capacities are pH-dependent, with maximum binding at pH near the protein isoelectric point.
An additional three peaks were found at convergent SSRs, which were not always scored as significant ORC1/CDC6 binding sites (e.g., OBR:8 1.89), consistent with our suggestion that the initiator binds transcription termination sites.
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