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A consequence of the existence of extended 3'UTR transcripts is that probe sets which were thought of binding at the 3' end may bind more upstream of the transcript.
To enhance visibility, noise reduced results for all cells are found in figure 4. The curves have been normalized to have a 100% binding at the end of incubation 2 to make comparisons possible.
Cleft closure accompanies strong actin binding at the end of the myosin power stroke.
To explain this, we proposed that enhanced HK2 binding at the end of ischaemia maintains functional contact sites that allow faster rates of PCr synthesis in the cytosol and thus more rapid regeneration of the cytosolic ATP.
These data confirm the extremely strong association between mitochondrial HK2 binding at the end of ischaemia and heart recovery during the reperfusion period, and suggest that pre-ischaemic glycogen content and rates of glycolysis during ischaemia play an important role in modulating HK2 binding through changes in [G-6-P] and pH.
The presence of G or C bases within the last five bases from the 3' end of primers (GC clamp), which helps promote specific binding at the 3' end, was taken into account.
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The objective function was to minimize the total amount of Gd in the core together with the residual Gd reactivity binding at the End-of-Cycle (EOC).
Experts noted that leaders were working to temper expectations since the overblown promises before Copenhagen nearly ended in a fiasco — with no binding agreement at the end.
"If Yvo de Boer thought that there would be a legally binding treaty at the end of this year, I suspect he would be sticking around to take some of the credit for it," said Michael A. Levi, an expert on climate change at the Council on Foreign Relations.
In fact, Apaf-1 IRES is also known to have two potential PTB binding sites at the 3' end region [13].
We examined the effect of binding parameters at the 3' end of miRNAs by constraining the total number of mismatches and GU pairs at nt positions ≥15 to be <6 (Figure 6).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com