The signal for cell proliferation is generated by intrinsic EGF-receptor (EGFR) tyrosine kinase activation via EGF binding at the cell surface, and terminated by receptor/growth factor complex internalization and degradation.
One member of this family, the lectin XCL from Xerocomus chrysenteron, induces drastic changes in the actin cytoskeleton after sugar binding at the cell surface and internalization, and has potent insecticidal activity.
In primary neurons we observed sAPP-Fc binding at the cell surface (not shown) but we have yet to identify molecular partners for this binding.
However, edelfosine treatment of Jurkat cells induced clusters of lipid rafts, identified through CTx B subunit binding, at the cell surface (Figure 2A).
As might be expected, the absence of capsule promoted robust plasminogen labeling, while the presence of capsule was sufficient to prevent plasminogen binding at the cell wall surface.
Taken together, these data indicate that overexpression of LARGE in DG-deficient neural stem cells increases laminin binding at the cell surface, suggesting that non-α-DG targets of LARGE-mediated glycosylation also mediate laminin binding upon overexpression of LARGE.
The HN protein contains the unique motif N-R-K-S-C-S at aa positions 234 239, which is thought to be involved in sialic acid binding at the cell surface [35], [36].
Using an antibody to the C-terminal V5 Tag on soluble RAET1G in FACS, NK cells cultured with RAET1G2 containing media for 1 hour showed a slight shift, indicating presence of RAET1G2 protein binding at the cell surface This was not seen in NK cells cultured for 24 hours (Figure 8C).
However, it may be possible to exploit avidity effects for the membrane-bound receptor, giving tighter binding at the cell surface than to the soluble receptor in solution.
This suggests that Kaiso binding at the cell type-specific sites may occur by a different mechanism than at sites common to both cell types.
