Only four of the eight different subunits bind ATP at physiological concentrations.
At physiological concentrations, binding of FGF23 to target cells requires co-expression of the ubiquitously expressed FGF receptor-1c and of αKlotho (Urakawa et al, 2006), hereafter referred to as Klotho.
BA has been shown to bind to cADPR at high concentrations, however, BA's ability to inhibit cADPR, caffeine and 4CmC induced Ca2+ release indicates that at physiological concentrations BA may bind to the cADPR binding site on the RyR stabilizing the Ca2+ channel in its inactive state [30].
Ascorbate binds to the beta 2 adrenergic receptor at physiological concentrations.
PEP binding was not affected when up to 1 mM MgADP was added to the assays, suggesting that MgADP does not bind to the N59D/A158T/S215H variant of TtPFK at physiological concentrations.
To compare how the lipid binding profile shown in Figure 4 compares to a situation in which anionic charges are dispersed and present at physiological concentrations in liposomes containing other lipids, we repeated the lipid binding assay using liposomes (for a subset of anionic lipids identified in Figure 4).
Altogether, the results reported in Fig. 7 strongly suggest that SLPI modulates, at physiological concentrations (20 150 µg/ml) [6], [7], the CD4-PLSCR1 interaction by competitive binding to the CD4-binding region of PLSCR1.
Binding of both nucleotide triphosphates (NTPs) to the immobilised proteins in the SPR assay was observed at physiological concentrations in the presence of Mg2+, which was present at 10 mM in the running buffer, whilst binding of cGMP only shifted from the baseline at concentrations higher than 1 mM.
Aggrecan mimics were cytocompatible at physiological concentrations.
By changing generator-collector spacings, the required performance at physiological concentrations should be possible.
