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Exact(1)
Except for PSK-CG, 5′FGUUUGUUC3′ was the competition strand, i.e., the same as for PSK 2. For PSK-CG, the competition strand was 5′FCGUUUGUUC3′, to allow saturated binding at convenient concentrations of the pseudoknot.
Similar(59)
The binding at physiological concentrations and higher (ca. 50 uM) occurs in the 190 210 nm range.
At both concentrations, CREB1 increased GABPα-GST binding, but this enhancement is less than that observed for GABPα-GST binding at high concentrations (30 ng), indicating that GABPα-GST binding at 2.5 ng to the canonical motif is not saturating.
Bortezomib (Velcade® PS-341, 20), a proteasome inhibitor, inhibits p300-HIF-1 α binding at nanomolar concentrations.
F54A abolishes binding, whereas H67Q does not affect binding at the protein concentrations shown.
Specific binding was calculated by subtracting nonspecific from total binding at each concentration of radioligand.
Its binding at the lowest concentration exceeded that of HGF at the highest concentration.
[18F]FBu-AMC13 only showed discernible specific binding at the lowest concentration tested.
The binding interactions with serum albumins (HSA and BSA) were performed with all these molecules and all of them show very strong binding at sub-micromolar concentration.
Here, we also observed more binding at lower salt concentration (Figure 8B).
At the same time, the convenient concentration of so much human frailty has transformed the hotel into a living museum of sad stories.
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