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These findings further validate our Myc binding assignments and indicate that the extent of binding is dependent on c-Myc levels.
The TF-gene binding assignments were provided in the form of binding p-values, on the hypothesis that the TF binds to the promoter region of the target gene.
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The errors in the quantitative analysis (relative concentration) were estimated in a range of ±5% and the accuracy of the binding energies assignments was ±0.2 eV.
The use of the modified Auger parameter for Cu and Zn species allows for corroborating evidence when there is uncertainty in the binding energy assignment.
The technique enables determination of binding constants, assignment of the type and number of ligand binding modes.
MochiView does not supply a comprehensive binding region assignment algorithm (a more limited peak extraction/refinement utility is provided), as approaches to calling binding regions are constantly being refined; moreover, the approaches for calling peaks vary with the platform used to analyze the precipitated DNA.
Additional characterization of the reductively methylated proteins used in this study, as well as further information on resonance assignments, ATP binding, and ligand titration.
Two-dimensional H N correlation experiments allow one to monitor the specific residues that contribute to inhibitor binding using previous backbone assignments for Aβ42.
The XPS curve-fitting program, XPSPEAK 4.1, was used for peak de-convolution and assignment of binding energies, which was referenced to the adventitious C1s peak at 284.6 eV.
In the interface-to-channel binding step, the channel assignment order of links should be determined first, which is achieved by sorting links in ascending order using the expected interference level (EIL) defined in Equation 12, where A l is a set of links that have already been assigned a channel.
This resulted in an average increase of the ROC-AUC from 0.52 to 0.62 (Table 1, Table S3) and underscores the importance of correct binding site prediction and assignment for receptor-based virtual screening [31].
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