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The binding assays were resolved by electrophoresis on 5% non-denaturing polyacrylamide gels.
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In order to validate the binding ability, ligand binding assays were established [20].
All binding assays were repeated three times.
All binding assays were done in duplicate.
Annexin V binding assays were performed as described earlier [9].
Visual MT binding assays were performed essentially as described [53].
Erythrocyte binding assays were performed as described earlier [23].
All binding assays were done in triplicates.
All binding assays were performed in triplicates.
In the O1s XPS spectra, two chemical binding states were resolved: (1) O2--M fully oxidized states (530.5 eV) and (2) OH (532 eV).
In the O1s XPS spectrum, two chemical binding states were resolved: (i) O-metal (530.5 eV) and (ii) OH-M (532 eV) binding states (where M represents In, Ga, and Zn); these binding states agree with those reported in many previous reports[16, 17].
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