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All quantitative data obtained by either radioactivity or fluorescence competition binding assays were included.
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A total of 70 samples positive by IHC, 33 positive by estrogen binding assay, 43 negative by IHC and 20 negative by estrogen binding assay were included in the current study (Tables 1, 2, 3 and 4 respectively).
A clonogenic assay was included.
In order to validate the binding ability, ligand binding assays were established [20].
All binding assays were repeated three times.
All binding assays were done in duplicate.
Annexin V binding assays were performed as described earlier [9].
Visual MT binding assays were performed essentially as described [53].
Erythrocyte binding assays were performed as described earlier [23].
Protein-DNA binding assays were performed as described [37].
Polysaccharide binding assays were performed as described [68] with modifications.
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