Sentence examples for binding assays were generated from inspiring English sources

The RNA transcripts used for the cleavage and binding assays were generated by T7 RNA polymerase using oligonucleotides as templates.

Finally, 100 possible binding conformations were generated by AutoDock run.

The metal binding states were generated at pH 7.0 by Epik [48, 49].

Next, the different binding levels were generated by varying k3 from 0.2 to 2.6 min−1.

The binding curves were generated assuming a single binding site curve fits using the Graph Pad Prism software.

Oligonucleotides corresponding to Tcf21 promoter with or without SRY binding site were generated by PCR amplification with primers listed in the Table S3.

Promotor region segments of human collectrin containing putative HNF-1 binding site were generated by PCR and cloned into pGL3 basic reporter vector (Promega)[13].

All the designed mutant nucleotide sequences were re-analysed in Match 1.0 Public to check for loss of the specific transcription factor binding site and that no new binding sites were generated.

A binding p-value was then determined for each genomic position by Wilcoxon rank sum test and binding sites were generated from those more significant than specified thresholds with a maximum gap of 500 and minimum run of 350.

Twenty different binding poses were generated per molecule and ranked according to their binding energies.

SHAPE signatures for each Rev binding event were generated based on EMSA results.