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TR-GATA-2 binding assays were analyzed considering the simple two-state reversible equilibrium between TR as described elsewhere [53].
Data from in vitro functional binding assays were analyzed by non-linear regression with a sigmoidal curve with variable slope to determine EC50 and IC50 values.
Afterwards, radioactively labeled proteins were detected after Coomassie blue staining by autoradiography while non-radioactive binding assays were analyzed by Western blot analysis.
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Results of the virus-turkey erythrocyte binding assay was analyzed by one-way ANOVA using the mean square in the ANOVA to estimate of variability.
The binding properties were analyzed in a competitive cell binding assay followed by internalization experiments in human PSMA expressing LNCaP cells.
The binding properties were analyzed in competitive binding, internalization, and cell surface retention experiments.
The binding kinetics were analyzed by BIAevaluation software (Biacore) using a 1 1 Langmuir binding model.
Data on protein levels and DNA binding activity were analyzed statistically by ANOVA using SAS programs.
Ligand binding data were analyzed using GraphPad Prism 4.03 (GraphPad Software , Inc.
Autoradiograms from ISH and receptor binding studies were analyzed using the optical density readings (grey intensity).
The binding data were analyzed by a mathematical calculation based on the 1∶1 (Langmuir) binding model (Table 1).
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