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To assess the binding site targeted by [18F]-9 on AD homogenates, binding assays were also performed in the presence of unlabeled analytically characterized samples of PiB, IMPY, and Chrysamine G.
The binding assays were also performed using naked beads to account for non-specific UBXN7 binding to the beads.
Improvements over the original starting polymer were a 6 times lower K50, which corresponds to higher affinity, 20% higher capacity at low analyte concentration (B2), 40% higher capacity (Bmax) and 1.3 times increased imprinting factor (IF). Binding assays were also performed in aqueous solvents.
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Two Myb binding motifs were also identified, and their role in regulating transcription was examined by mutation and functional testing.
Blank, growth controls, negative control and binding control were also assessed.
Many binding sites were also manually curated from literature sources.
Importantly, all previously identified (microsatellite) binding regions were also found.
Binding studies were also conducted for selected mutants.
Several large-scale experimental binding studies were also examined to identify binding sites [ 2, 32, 226- 229].
REES changes due to oxomalonate binding were also observed with the αγ and α subunits.
Molecular functions relating to ATP binding were also enriched (P < 0.003).
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