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With G-actin and F-actin binding assays, we identified a CapG nanobody that binds with nanomolar affinity to the first CapG domain.
Using ELISA-type solid phase binding assays, we identified a F3-binding site to a region between BBK32 residues 146 and 205 (data not shown).
By analyzing results of reported DNA binding assays, we identified several CpG rich motifs as potential MLL1 binding units (defined as morphemes).
Using isothermal titration calorimetry (ITC -based quantITC -basednding assays, we identified a 48-residue auto-inhibitory segment (residues 1577–1624, referred to as 'AS') as the complete ANK repeat-binding region.
Similar(56)
Using protein-lipid overlay and liposome-binding assays, we identified that the N-terminal region of Dab2, including its PTB domain (N-PTB), specifically interacts with sulfatides.
Using a far western approach followed by in vitro peptide-binding assays, we identified two Nck-binding peptides with PXXPXR class II SH3-binding motifs in N-WASP.
We used a two-step strategy: Firstly, by a competition binding assay, we identified the proper boundaries of the domain recognized by 2F5, which we found considerably larger than the ELDKWAS hexapeptide.
Using promoter assays, Northern blot and ChIP (chromatin immunoprecipitation) assays, we identified that E2F-1 couldirectlyly transactivate TSP1 expression by binding to its promoter, further expending TSP1's regulatory factors.
Using a lectin-binding assay, we identified Erythrina cristagalli agglutinin (ECA), which particularly recognizes the β-galactoside structure on the surfaces of MIN6-m9 cells.
Although our current study did not include any direct measurements and comparisons of bitter taste perception in the white-throated sparrow nor any allele-specific ligand-binding assays, we did identify nonsynonymous variants in five of the intact white-throated sparrow TAS2Rs, suggesting that there is the potential for variation in bitter taste perception in the white-throated sparrow population.
Using a plate-based, fluorescence polarization (FP) assay, we identified a minimal region of LZ4 that suppresses binding of HSF1 to the HSE.
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