Sentence examples for binding assays using the from inspiring English sources
The percentages of beta 1- and beta 2-adrenergic receptors were determined in three cocaine users and four control patients by performing competition binding assays using the beta 2 antagonist ICI 118,551.
In vitro binding assays using the CTCF target motif at the IGF2BP1 gene, as well as allele-specific analysis of cytosine methylation and CTCF binding, revealed that CTCF does not regulate mono- or biallelic IGF2BP1 expression.
Binding assays, using the truncations as probes on insect protein blots, revealed that the actin-binding region of PGK was located on the truncated peptide designated PGK-FL5 containing amino acids 49 154.
Binding assays, using the Escherichia coli FabF C163Q mutant, which mimics the acyl-enzyme intermediate9, revealed that the dissociation constants (KD) for 1 and 2 were ~2-fold tighter than those of 3 and 4 (Table 2 and Supplementary Figs. 38b and 39).
Binding assays using the fluorescence reporter N-phenyl-1-naphtylamine (NPN) were inconclusive.
The CXCR4 receptor affinities of [68Ga]pentixafor and [68Ga]NOTA-pentixafor were determined in competitive binding assays using the leukemic CXCR4-expressing Jurkat T-cell line and [125I]FC131 as the radioligand.
A receptor binding assay using the fhER-α ligand-binding domain showed that alkylphenols bind to fhER-α 50 times more efficiently than to human ER-α (Urushitani et al. 2003).
To determine the extent of functional αIIbβ3 integrin expression that occurs on platelet activation, we performed a binding assay using the PAC-1 monoclonal antibody, a fibrinogen mimetic that only binds to the activated conformation of the αIIbβ3 integrin.
The resulting [3H]isoVa-RYYRIK-NH2 was evaluated in a saturation binding assay using the COS-7 cell membrane preparations of transiently expressed ORL1.
B16 cells were cultured with or without 1 µM ATRA for 24 h, and were subjected to EGCG binding assay using the surface plasmon resonance (SPR) biosensor SPR670 (Moritex Corp., Tokyo, Japan).
