Sentence examples for binding assays using a from inspiring English sources
Assembled complexes can be used in high-throughput DNA strand transfer assays if radio labeled target DNA is employed or in integrase binding assays using a suitable radioligand.
The interaction was further confirmed by in vitro binding assays using a GST-hnRNP-K fusion protein bound to glutathione-sepharose 4B beads.
Competition ligand binding assays using a range of ligands with A2AR SMALP were consistent with established A2AR pharmacology (International Union of Pharmacology (IUPHAR) database) and comparable to A2AR binding in the original yeast membranes (Table 1).
To test whether the spliceosomal RNAs bind nonspecifically, we repeated the binding assays using an N-terminally MBP-tagged MS2 dsRNA binding protein (MBP-MS2BP) that interacts with the MS2 RNA hairpin loop.
Briefly, uptake and binding assays used a high sodium, low potassium buffer containing glucose and tropolone, and the non-specific binding was defined with 1 μM CFT as in our previous work.
The GAG content of each sample (n = 3) was quantitated by using a 1, 9-dimethylmethylene blue (DMMB, Sigma) dye binding assay, using a standard curve generated by chondroitin sulphate B. The absorbance was read at a wavelength of 525 nm.
This assay is a single-step primary binding assay using a single reagent - the QD-labeled antigenic peptides.
To screen chemical libraries for active molecules we developed an in vitro fluorescence microscopy-based binding assay using a recombinant Ndc80 complex and taxol-stabilized MTs.
In addition, the DNA binding ability of C4 protein was proved to be unspecific by protein-DNA binding assay using a 1 kb DNA ladder of linear dsDNA fragments.
For example, a competition binding assay using a fluorescent antagonist for A3 receptor (Table 1 and 2) and a high-content screening system for the automated capture and analysis of images was developed.
