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All the compounds were screened in competitive radioligand binding assays to determine their affinities for μ- MOR), and κ-(KOR) opioid receptors.
A new series of 5-methyl-thiazolo[5,4-d]pyrimidine-7-ones 5-methyl-thiazolo[5,4-d]pyrimidine-7-ones 5-methyl-thiazolo[5,4-d]pyrimidine-7-ones 5-methyl-thiazolo[5,4-d]pyrimidine-7-ones 5-methyl-thiazolo[5,4-d]pyrimidine-7-ones 5-methyl-thiazolo[5,4-d]pyrimidine-7-onesdenosine receptors (ARs).
We next performed ELISA-based binding assays to determine the ability of the fiber-incorporated zipper domains to form heterodimers with the corresponding zipper-scFvG28-5 species.
We used equilibrium binding assays to determine the binding affinity of recombinant traps for VEGF.
The two most potent blockers (compounds 9 and 10) were then characterized using radioligand binding assays to determine their affinity for CB1 and CB2 receptors.
Biotinylated KU-174, a potent Hsp90 C-terminal inhibitor, was used for drug protein binding assays to determine how the C-terminal inhibitors disrupted Hsp90α/Aha1 interactions.
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Additional file 1: Supplementary materials including methods and results of a receptor binding assay to determine the affinity of [11C]HMS011 for human AMPA receptors and supplementary figures.
Routine quality control of the labeled antibody was performed using instant thin layer chromatography to estimate the radio-purity and cell binding assay to determine the immunoreactivity.
This extract was used for a solid-phase binding assay to determine potential binding with Aβ1-42 and Aβ1-16 using modification of a previously described protocol [ 26].
To test this, we used an in situ chromatin binding assay to determine chromatin association of Pcs1 in single cells [21].
We used a whole-cell IrAM binding assay to determine the relative affinities of AM, AM2 and hαCGRP at the AM1 receptor.
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