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Indeed, competitive binding assays demonstrated that ponatinib could directly bind RIPK1 i n vitro with high affinity (Kd: 37 nM).
The binding assays demonstrated that rMsEno was able to bind to chicken Plg and human Fn proteins.
In vitro cell binding assays demonstrated that the A33scFv-Fc produced by P. pastoris can bind GPA33-positive but not GPA-negative tumor cells.
Quantitative in vitro binding assays demonstrated that RBR-2 is required for high affinity binding of EIAV Rev; ERRE fragments containing both RBR-1 and RBR-2 have much higher affinity for EIAV Rev than those lacking RBR-2 (Figure S1).
Moreover, in vitro peptide binding assays demonstrated that the affinity of 14 3 3 for S28 phosphorylated peptides and H3S10K14 phosphoacetylated peptides is very similar [23].
Studies using knock-in mice and in vitro binding assays demonstrated that the T192M mutation affects the LG-binding modification of α-dystroglycan by disrupting its interaction with LARGE (i.e., formation of the enzyme substrate complex).
Similar(52)
Characterization of the 23 myosin-II monoclonal antibody binding sites by antibody staining of one-dimensional peptide maps and solid phase, competitive binding assays demonstrate that they bind to at least 15 unique sites on the myosin-II heavy chain.
Moreover, kinetic-binding assays demonstrated that the sites were linked by negative heterotropic allostery.
Competitive binding assays demonstrated no advantage in terms of receptor affinity through dimerization.
In vivo DNA binding assays demonstrate that VNG0258H binds DNA to mediate gene regulation.
In vivo binding assays demonstrate that VNG0258H binds directly to the promoter of sod2, encoding the [Mn] superoxide dismutase.
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