Sentence examples for binding assay with the from inspiring English sources

In order to confirm that honokiol indeed binds to PPARγ we performed a competitive FRET-based ligand binding assay with the purified human PPARγ LBD.

Detection of estrone, 17β-estradiol and BPA was achieved by integrating a competitive binding assay with the piezoelectric sensor.

That result could be concluded from the filter binding assay with the highest concentration of aptamers available, since there was a large number of bound aptamers.

Testosterone, LH, and FSH values were assessed using a one-step competitive binding assay with the Beckman Coulter Access II Immunoassay system (Beckman Coulter, Inc., Brea, California).

To investigate whether the different agarose binding behavior of AID could be explained by the presence or absence of additional proteins that AID forms complexes with, we performed size exclusion chromatography of AID positive cell lysates and did an agarose binding assay with the fractionated high molecular mass (HMM) and low molecular mass (LMM) AID protein complexes (Figure 4A).

In addition, ML171 did not significantly bind to a large battery of human or rodent G-protein coupled receptors (GPCR), channels and transporters expressed in the central nervous system, did not significantly bind most of the receptors tested in the binding assays with the exception of serotonin (5-HT2B and 5-HT2C), adrenergicrgic (alpha2C) receptors (% of inhibition >60%).

Thus, we plan on performing binding assays with the above candidate molecules in CML cell lines and primary CML cells.

The initial binding assays with the synthesized sequences named KDED1 to KDED20 revealed many sequences binding to the cell target (Figure 2).

Isothermal DNA binding assays with the TR and GATA-2 were carried out using an ISS-PC1 spectrofluorimeter (ISS, Champaign, IL), assembled in "L" geometry.

First, we prepared cell membranes from cultured neonatal cardiomyocytes and performed radioligand binding assays with the muscarinic receptor antagonist N-methylscopolamine.

Competitive DNA binding assays with the same set of substrates as used for the experiments with GFP-CXXCDnmt1 and GFP-CXXCTet1 reported above (Figure 2C) showed that both GFP-Dnmt1 and GFP-Dnmt1ΔCXXC bind DNA independently of the presence and methylation state of a CpG site (Figure 4B).