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In vitro competitive binding assay was performed using [3H]CP-55,940 in U87hCB1 and U87hCB2 cells.
ELISA binding assay was performed on native TTCF (green), native TTCF heated for 2 h at 80 °C (red), TTCF release from ensilication (purple) and ensilicated TTCF heated for 2 h at 80 °C and released (blue).
In order to determine the binding affinity, a competitive cell binding assay was performed.
A sigma-1 receptor binding assay was performed using the following method.
The virus binding assay was performed using a previously reported protocol, with modifications (Qing et al., 2016).
A binding assay was performed as described previously [24].
Similar(6)
GTP-binding assay was performed as previously described [20], [21].
The [H]Ry-binding assay was performed as previously described (Pessah et al. 1987).
The actin monomer-binding assay was performed as described earlier [ 20].
In vitro RNA-binding assay was performed as described by Ohman (Ohman et al, 2000).
The DNA-binding assay was performed by using the end-labeled DNA oligodeoxynucleotide of 32 bases with the sequence 5'-TTCGAGCTCGGTACCCGGGGATCCTCTAGAGT-3'.
More suggestions(18)
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binding reaction was performed
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