Sentence examples for binding assay was confirmed from inspiring English sources

The identity of several proteins recruited in our in vitro binding assay was confirmed by MS/MS.

The preference for Cdc5-D3 binding to double-stranded RNAs detected in the in vitro binding assays was confirmed through chemical shift perturbation by examining the HSQC spectra of N-labeled Cdc5-D3 afteRNANA was added at a 1 1 protein-to-RNA molar ratio.

The binding improvement over the amine-coupling system shown by assay was confirmed by a separate surface plasmon resonance experiment.

The results of internalization assay was confirmed by MTT assay.

Radioligand competition binding assay was carried out to confirm binding of the six hits to the β2AR using [I]cyanopindolol with crude membrane fractions containing the overexpressed β2AR (Supplementary Figure S2).

Next, a solid-phase binding assay was carried out to further confirm interactions between FtsZ and ClpX.

The importance of these WT1 and SP1 TFBS as candidate binding sites was confirmed by the in vivo ChIP assay.

The binding mode was confirmed by X-ray crystallography and assays with the MetAPs from Escherichia coli, Staphylococcus aureus and both human isoforms.

Specificity of NF-κB binding was confirmed by competition assays and the ability of a specific antibody to supershift protein-DNA complexes.

The essential role of Phe313 and Trp305 in N-6 binding was confirmed by our SPR assays, showing that N-6 had much lower binding affinity to two RXRα-LBD point mutants with Ala substitution of Phe313 or Trp305 (Fig. 4D and 4E).

As expected, FEZL bound to the 5UTR region of IGF1R, and the specificity of binding was confirmed by gel supershift assays using the specific antibody.