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A receptor binding assay using the fhER-α ligand-binding domain showed that alkylphenols bind to fhER-α 50 times more efficiently than to human ER-α (Urushitani et al. 2003).
To determine the extent of functional αIIbβ3 integrin expression that occurs on platelet activation, we performed a binding assay using the PAC-1 monoclonal antibody, a fibrinogen mimetic that only binds to the activated conformation of the αIIbβ3 integrin.
The resulting [3H]isoVa-RYYRIK-NH2 was evaluated in a saturation binding assay using the COS-7 cell membrane preparations of transiently expressed ORL1.
B16 cells were cultured with or without 1 µM ATRA for 24 h, and were subjected to EGCG binding assay using the surface plasmon resonance (SPR) biosensor SPR670 (Moritex Corp., Tokyo, Japan).
The array was used to perform a protein protein binding assay using the SUMO2 paralog.
We performed in vitro binding assay using the A-box-mutated SINE element as described before (Britten, 1996; Toth and Biggin, 2000) of CDKN1A").
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The CXCR4 receptor affinities of [68Ga]pentixafor and [68Ga]NOTA-pentixafor were determined in competitive binding assays using the leukemic CXCR4-expressing Jurkat T-cell line and [125I]FC131 as the radioligand.
This expression system exhibited sufficient production for 500 binding assays using the radiolabeled compound, [ S]GTPγS.
First, binding assays using the fluorescence of a single-tryptophan derivative indicate that As(GS 3 binds to the enzyme much faster than inorganic As III).
In vitro binding assays using the CTCF target motif at the IGF2BP1 gene, as well as allele-specific analysis of cytosine methylation and CTCF binding, revealed that CTCF does not regulate mono- or biallelic IGF2BP1 expression.
The binding assays using the full-length and several truncated versions of CRE1/AHK4 (Fig. 2) confirm the hypothesis that trans-zeatin is bound by CRE1/AHK4 via the CHASE domain.
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