Sentence examples for binding assay using a from inspiring English sources

The GAG content of each sample (n = 3) was quantitated by using a 1, 9-dimethylmethylene blue (DMMB, Sigma) dye binding assay, using a standard curve generated by chondroitin sulphate B. The absorbance was read at a wavelength of 525 nm.

This assay is a single-step primary binding assay using a single reagent - the QD-labeled antigenic peptides.

Induction of apoptosis was studied by annexin V-FITC binding assay using a kit from Beckman Coulter.

To screen chemical libraries for active molecules we developed an in vitro fluorescence microscopy-based binding assay using a recombinant Ndc80 complex and taxol-stabilized MTs.

In addition, the DNA binding ability of C4 protein was proved to be unspecific by protein-DNA binding assay using a 1 kb DNA ladder of linear dsDNA fragments.

This raises the possibility that substrate phosphorylation site inhibitors identified from a competitive binding assay using a bisubstrate probe may be ruled out as ATP-competitive inhibitors during counter screening.

For this, we performed an in vitro binding assay using an unmodified or a sumoylated 35S-labeled DRIL1 with recombinant GST-E2F1 or GST protein as a control (Fig. 5A).

To confirm that HEMADO was still capable of binding to the A3 W243F-YFP receptor, we investigated the affinity of NECA and HEMADO at both A3-YFP and A3 W243F-YFP receptors using a previously characterized fluorescence-based competition binding assay, using an xanthine amine congener-based fluorescent antagonist, CA200645 (Stoddart et al., 2012).

Assembled complexes can be used in high-throughput DNA strand transfer assays if radio labeled target DNA is employed or in integrase binding assays using a suitable radioligand.

In order to study the molecular interactions to α-TTP, theoretical binding studies by means of docking techniques and experimental binding assays, using a fluorescent probe, were performed.

Further binding assays using a panel of reference glycosphingolipids showed a specific interaction between the F6 fimbriae and a number of neolacto core chain (Galβ4GlcNAc) glycosphingolipids.