Sentence examples for binding assay to test from inspiring English sources

We used a Far Western ligand binding assay to test the ability of the PBD to bind NPM/B23.

Solid-phase radiolabeling binding assays to test IG3 ability to bind to its targets.

Examples of this approach include washing cell lysate extracts over a bead column fixed with an approved drug [ 64], high-throughput Biacore screening of an approved drug library against protein tyrosine phosphatase 1B [ 65], or high-throughput direct-binding assays to test drugs against 317 kinases [ 19].

To demonstrate whether the action of E2F1 on TSP1 occurs in vivo, we conducted chromatin Immunoprecipitation assay to test the binding of E2F1 in the TSP1 promoter.

Further, we performed ChIP assay to test the binding of OCT1 to synbindin promoter in vivo.

We have developed a straightforward pull-down assay to test ATP binding capability, and used N-glycosylation mutagenesis both to confirm the extent of N-linked glyconylation on hP2X4 and DdP2XA and validate homology models of the two receptors.

To test the direct interaction of miRNAs with the 3'UTR, we developed a PCR assay to test the potential binding interactions of the miRNAs with the 3'UTR and to validate the target sites in silico.

The protein kinase selectivity profile of RO9021 was assessed by the widely accepted KinomeScan method, which utilizes a proprietary active site-directed competition binding assay to quantitatively measure interactions between test compounds and more than 450 human kinases and disease-relevant mutant variants.

To substantiate this finding, we took advantage of chromatin immunoprecipitation (ChIP) assays to test whether the binding of NF-κB to its endogenous promoter was influenced in LRP16-knockdown cells.

In traditional approaches, investigators have used luciferase reporter assays to test individual TF binding sites of enhancers.

Peptides predicted to bind were synthesized and tested in binding assays to determine those with high affinity.