Sentence examples for binding assay on a from inspiring English sources

Highly sensitive measurement of AFP-L3 was achieved using a microchip capillary electrophoresis and liquid-phase binding assay on a μTASWako i30 autoanalyzer (Wako Pure Chemical Industries, Ltd).

The measurements of hs-AFP-L3% and DCP were achieved by using a microchip capillary electrophoresis and liquid-phase binding assay on a μTASWako i30 auto analyzer (Wako Pure Chemical Industries, Ltd, Osaka, Japan) (Kagebayashi et al, 2009).

The concentration of aPKC was determined by comparing its reactivity with an anti-aPKC antibody (Santa Cruz Biotechnology) with that of a standard at a known concentration (bacterially expressed aPKC kinase domain purified and quantified using a Bradford dye binding assay) on a Western blot.

Serum DCP levels were determined using a sensitive enzyme immunoassay (Eitest PIVKA-II kit, Eisai Laboratory, Tokyo, Japan) according to the manufacturer's instructions 20– 22. Conventional measurement of AFP-L3 was performed using a column chromatography and liquid-phase binding assay on a LiBASys autoanalyzer (Wako Pure Chemical Industries, Ltd., Osaka, Japan) 23, 24.

Finally, a diverse subset of 15 structurally new compounds (compounds 1– 15), which were not previously tested for α7 receptor activity, was selected for binding assays on a chimeric α7/5-HT3 receptor (Figs. 4 and 5).

The binding affinity of [natGa]3 was compared to c RGDfK) 2 in a competitive binding assay on αvß3-positive U-87 MG cells using [125I]I-echistatin as radioligand.

The affinity of the three 125I-radiolabeled 14C5 chAb derivatives was compared in a saturation binding assay on A549 cells (Figure 4).

With the goal of targeting the human bladder using phage display technology we designed and tested a tissue binding assay on intact urothelium ex vivo.

The binding affinity of FPyPEGCBT-c RGDfK) was evaluated using a standard in vitro binding assay on U-87 MG and SKOV-3 cancer cells expressing αVβ3, αVβ5 and α5β1 integrins and compared to a reFPyPEGCBT-c RGDfK(RGDfV) (Fig. 2).

It has been demonstrated that curcumin modulates the activation of the transcription factor nuclear factor- κB (NF- κB) for this reason, we performed a nuclear NF- κB DNA binding assay on MIA PaCA-2 cells treated with curcumin and controls, and we demonstrated that curcumin downregulated NF- κB activation in MIA PaCa-2 cells).

CCPA showed a Ki value of 4.2 nM in the binding assay on rat cortical brain membranes (Fig. 3D).