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A detailed description of the PPARγ binding assay is provided in Supplemental Material, "PPARγ Competitive Binding Assay and Quality Assurance/Quality Control".
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Further strong evidence for the biological relevance of the results of TAP binding assays was provided in a study showing that TAP binding affinity paralleled closely the efficiency of epitope presentation by cell surface class I molecules [17].
Results of competitive ligand binding assays are provided in Table 1.
An example of binding events for each step of the protein expression profiling assay is provided in Figure S3.
GST-Nck1 SH2 and GST-Nck2 SH2 expression clones for in vitro binding assays were kindly provided by Gianni Cesareni and Bruce Mayer [16].
The in vitro pVHL-HIF-2α binding assay was performed according to a previously published protocol67.
A competitive flow cytometry-based binding assay was performed to further validate the identified hCXCL1 binding epitopes in different ELR+ CXC chemokines.
A competitive binding assay was also performed using unlabelled DNA target to demonstrate its applicability to real sample analysis.
The binding assay conditions and radioligand binding assay are highly valuable to identify and design better HIV inhibitors for HAD.
In order to determine the binding affinity, a competitive cell binding assay was performed.
To determine if adenine nucleotides directly contribute to the regulation of GRP94 peptide binding activity, an in vitro peptide binding assay was developed.
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