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Cyclic AMP Binding Proteins (cAMP-BP) levels have been measured by means of a competitive binding assay in the cytosols of 50 human colorectal cancers.
Extended Data Fig. 8 PTCH1* SHH-N binding assay in the detergent-free system.
The binding affinity of [natGa]3 was determined by a cell-based competitive binding assay in the human glioma cell line U-87 MG with [125I]I-echistatin as the radioligand as previously described (Dumont et al. 2011).
Taken together, these results show that the αVβ3-specificity claimed for many RGD peptide tracers cannot be concluded from competitive cell binding assay in the U-87 MG model.
rRH5 was incubated with wild type erythrocytes in the erythrocyte binding assay, in the presence of different amounts of purified anti-RH5 IgG, from 0 10 µg IgG.
For the agarose binding assay in the presence of RNase (only in the case of transfected, exogenous AID), the samples were treated with RNase A (Roche) at concentrations of 0, 1 µg/ml (low), 100 µg/ml (medium), or 1 mg/ml (high) RNase for 1 hour at room temperature before incubation with agarose beads.
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Recent whole genome polymerase binding assays in the Drosophila embryo have shown that a large proportion of unexpressed genes have pre-assembled RNA pol II transcription initiation complex stably bound to their promoters.
In vitro affinities were derived from calcium influx binding assays in the presence of the calcium-sensitive dye Fura-2/AM in stably P2X7-transfected cells.
In vitro studies including radioreceptor binding assays in the rat brain membrane and cell uptake studies in the A375 cell line were performed.
To investigate this question, we performed agarose binding assays in the presence of increasing concentrations of RNase A (Figure 3A).
Sulfonamides did not displace biotinylated active site isostere in competitive binding assays in the absence of substrate.
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