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For that purpose, we designed a cell-free receptor binding assay consisting of a solid-phase recombinant soluble GM-CSF receptor α (GMRα) and a biotinylated GM-CSF (bGM-CSF).
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Each assay consisted of two allele-specific minor groove-binding (MGB) probes, labeled with fluorescent dyes VIC and FAM.
Each assay consisted of an allele-specific SuperSelective primer45 and a common primer shared by the normal and mutation assay.
The assay consists of a chip covered with DNA strands.
This assay consists of three chambers connected in unison.
The assay consisted of three separate trials.
Each assay consisted of a minimum of three independent homogenates.
VU0467154 was tested at a concentration of 10 μM in the Ricerca Lead Profiling Screen (Ricerca Biosciences, Taipei, Taiwan), a radioligand binding assay panel consisting of 57 GPCRs, ion channels, and transporters.
Unless stated otherwise, PCR assays consisted of 40 cycles.
The direct visualisation of PrPSc bound to soil particles was carried out using a novel immunodetection assay consisting in: (i) Saturation (1 h, RT) of the soil binding sites for proteins with bovine serum albumin (BSA, 5% in PBS).
Membrane-associated GTP-binding and/or GDP-binding signalling proteins consisting of three subunits α, β and γ.
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