Sentence examples for binding assay as previously from inspiring English sources
The resulting plasma membranes were suspended in binding buffer and used in [35S]GTPγS binding assay as previously described [11].
Receptor binding specificity of A/Quzhou/1/2015(H7N9) was analysed by a solid phase direct binding assay as previously described.
Plasmatic concentation of Ischemia modified albumin was determined by copper binding assay as previously described [ 7], using a microplate reader Infinite 200 PRO (Tecan, Switzerland).
GAD65, islet cell antigen (IA-2), and zinc transporter isoform 8 (ZnT8) autoantibodies were determined by radioligand binding assay, as previously described (8, 9).
The binding affinity of the lipopeptides for purified LPS was examined using our synthetic dansyl-polymyxin binding assay as previously reported.
At the end of 2nd, 3rd, and 4th week of the incubation periods, developed biofilms were quantified using the crystal violet binding assay as previously described by Stepanović et al. [ 23] and Adetunji and Adegoke [ 24] with some modifications.
The K d of [125I]-AB-MECA obtained from the novel kinetic real-time cell-binding studies and from traditional binding assays, as previously reported in the literature (see Table 2 for detailed summary of K d values), was compared using two-tailed, unpaired Student's t test with Welch's correction (d).
Inhibitory constants of the A3R antagonists MRS1191, MRS1523 and FE@SUPPY, respectively, were obtained from competitive real-time cell-binding studies at ambient temperature with 0.7 nM [125I]-AB-MECA on CHO-K1-hA3R cells (group A) and compared to the corresponding K i values obtained from traditional binding assays, as previously reported in the literature (group B).
Epidermal growth factor receptor levels (fmol mg−1 prot−1) were 33 800 and 6500 for CAL33 and CAL39, respectively (ligand-binding assay as previously published by us; Olivier et al, 1990).
