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In the case of Tnfrsf12a, regulation via κB proteins may involve a complex of p65 p50 Bcl-3 binding as previously described in response to TNFα [29].
Sections on grids were first quenched for non-specific antibody binding as previously described [70], [71], then reacted with primary rabbit anti-fiber or anti-penton base antibody used at a dilution of 1∶50 to 1∶500 in Tris-buffered saline (TBS) containing 1% bovine serum albumin (TBS-BSA), and incubated overnight at 4°C.
We deparaffinized lung sections and blocked nonspecific binding as previously described (Ghanem et al. 2004).
Samples were scored to determine the proportion of tumor cells exhibiting pimonidazole binding, as previously described [ 35].
Equilibrium Kd values for the binding of the TnH 30−61) and TnH(126−159) peptides were determined using competition with melittin binding as previously described (26).
The amounts of [H]Phe-tRNAPhe bound to the ribosome, representing ternary complex, were analyzed using glass microfiber filter (Whatman) binding as previously described [ 23].
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The resulting plasma membranes were suspended in binding buffer and used in [35S]GTPγS binding assay as previously described [11].
[H]HC-3 binding with mouse and rat brain membranes exhibited multisite binding kinetics, as previously described, precluding accurate assessment of kinetic parameters in native tissues.
Receptor binding specificity of A/Quzhou/1/2015(H7N9) was analysed by a solid phase direct binding assay as previously described.
The specific binding of Rad51 to the RECQ1-coated wells was analyzed according to Scatchard binding theory as previously described [39], and the apparent dissociation constant (Kd) was determined to be 11.9±2.1 nM.
The BN6, BN7, and BN8 peptides are modifications of the BN4, BN2, and BN5 peptides, respectively, in which a DPhe has been introduced at the N-terminus in order to increase binding affinity as previously described [11].
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