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Allele-specific differences of the predicted ΔΔG values, indicating strength of miR binding, are summarized in Fig 6A.
Available amino acid sequences of R. perezi, X. laevis and X. tropicalis were aligned (see Additional file 14) and key residues for substrate and coenzyme binding are summarized in Table 5.
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A comparison of SPR derived data and binding kinetics are summarized in Figure 2C The effect of BH4 (20 μM) on GCH1 GFRP interactions was also investigated, revealing increased binding affinity in its presence but no distinguishable differences between T-GCH1 GFRP T-GCH1 GFRPGFRP interandions (Supporting InF-GCH1 GFRPable F-GCH1 GFRP
All binding data are summarized in Table S1.
The results are illustrated in Figure 3 and the binding parameters are summarized in Table 1.
The binding constants are summarized in Table 1.
The bond lengths of the three different binding modes are summarized in Table 2 and are in good agreement with other TRIS-functionalized Anderson POMs.
The genes from each of the four lists that are near a putative MrpC binding site are summarized in Additional file 7.
Known and predicted membrane-binding sites are summarized in Figure 1A.> -wrap-foot> Helical wheel representations for these helices are in Additional file 1: Figure S9.
The binding affinity predictions are summarized in Figure 6.
Using label-free microscale thermophoresis, we successfully quantified different types of biomolecular binding events which are summarized in Table 1.
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