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In E. coli, the same NMR resonances that disappear upon Δ binding are not detected.
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This binding was not detected in female pupae 3 days before emergence.
Interestingly, although the inhibitory region peptide (TnI96 115) was observed to bind with an affinity similar to that of TnI96 131 by fluorescence analysis (Kd=380 nM), its binding was not detected by SPR.
Cell surface fiber binding was not detected following Ad4 infection, suggesting that the 4D2 mAb may not recognize Ad4 fiber molecules.
> -wrap-foot> "B" indicates binding was detected by affinity gel electrophoresis; "–" indicates binding was not detected.
Antibody binding was not detected using preimmune serum or with antiendokinin antiserum preincubated with an excess of immunogen peptide.
Furthermore, ORC binding was not detected at 23% of known ARSs, while nimACSs, which predict ORC binding, are found at 103 of 178 of the MCM2-only sites.
Conversely, even though open reading frames NGO1215 and NGO0546 have potential FNR binding sites upstream, FNR binding was not detected by ChIP.
As expected, Nanog binding was not detected in the extraembryonic endoderm (XEN) cell line where Nanog is not expressed ([ 8] and our unpublished data).
C139D/C147D protein folding was validated by thermal shift assay [ 14], but G-actin binding was not detected by ITC, indicating decreased affinity.
No binding was detected for 23 of the GFP_CBM constructs using the pull-down assay, and so are not included in Table 2.> -wrap-foot> "B" indicates binding was detected by pull-down assay; "–" indicates binding was not detected.
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