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Present work describes the design of a new one-pot hydrothermal route for the preparation of highly electroactive nanocomposite, SnS2 decorated reduced graphene oxide nanoribbons (designated as SnS2-rGONRs) for analytical and binding applications of a flavanone, naringenin (NAR).
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EARLY DECISION (ED) I AND II In this binding application program, a student applies on or before Nov. 1 (sometimes Nov. 15) and then receives his or her admission decision by the middle of December.
EARLY ACTION (EA) I AND II In this non-binding application program, a student usually applies EA I on or before the first of November (sometimes Nov. 15) and receives an admission decision by the middle of December.
In general, for a fixed geometry of the unperturbed system, the exciton binding energy is a decreasing function of the intense laser field parameter and of the dc electric field, although certain combinations of the two applied field intensities may lead to a rather insensitive behavior of the binding energy with respect to the application of a dc field.
The next step involved application of a methyl-binding protein capture protocol (MBD-cap) to capture methylated fragments from total fcDNA isolated from plasma.
The general steps for each of the staining procedures are similar comprising the consecutive steps of endogenous alkaline phosphatase or peroxidase inhibition using 0.2 N HCl or 0.5%H2O2O2 in methanol, respectively, followed by heat-induced epitope retrieval (HIER), blocking of slides to prevent aspecific antibody binding, and application of the appropriate antibodies and visualization methods.
This protection was completely reversed by co-application of a soluble EPOR capable of binding and chelating EPO.
Our calculations demonstrate that, in the case of Li-doped graphene, the application of a positive electric field yields an increase in binding strength during adsorption while a negative field decreases the binding strength during desorption.
Applications were shown on a small-molecule mixture and the binding of a dodecapeptide to a membrane mimetic.
Retained in this new construct is the ligand-induced structure-switching binding mechanism that is important in biosensing applications of the cocaine-binding aptamer.
The affinity of this mutein to biotin was high, comparable with that of tamavidin 2. These findings indicate that tamavidin-R104EK141E has the potential to serve as a robust tool in the numerous applications of biotin-binding proteins.
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