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The third model was based on the 20 positions related to mouse monoclonal antibody binding (Appendix) (9, 10).
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The analyzed fragment had 6 aa exchanges, but they were insufficient for drawing conclusions about protein function because the fragment did not include domains putatively relevant for receptor binding (Technical Appendix Figure 2).
The number of reads and AR binding events is shown in Appendix Table S4.
In this context we stress the fact that the non-specific background intensity is rather a variable contribution which progressively decreases with increasing amount of specific binding than a constant (see appendix A and the figure shown there).
The accumulated work values in Figure 9 place this study far outside of this regime, and it is likely the poor sampling of exponentially rare work values that lead to systematic errors and ultimately the disagreement between various protocol estimates of the binding energy discussed in the appendix.
The surface of PTEN that binds to membranes and the catalytic site that requires binding phospholipid head groups are known (Appendix figure 4A).
A cavity is formed below this loop and adds an appendix to the substrate-binding pocket.
At the protein side, only the appendix and the anticodon-binding module participate in the interaction between AspRS and the mRNA domain.
In addition, to make the scenario more convincing on the "translation from DRT to covalent binding" (considering the myopia of evolution), I add a new section in Appendices (Appendix 2) to address the problem concerning the original source of adaptors.
This linear relation can be derived from the ratio of the specific binding to the nondisplaceable binding using different reference regions (see Appendix).
For example, in appendix 1, the concatenation of several amino acid binding sites versus individual ones, does not significantly reduce the replication burden.
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