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Anti-TPO binding antibodies were also observed in a small proportion of healthy subjects (Fig. 2).
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Polyreactive gp41-binding antibodies were also isolated from uninfected individuals.
The antigen binding specificities of elicited antibodies were also similar and independent of the adjuvant used or the number of vaccine component alleles.
The binding properties of the resulting antibodies were also studied against their known homologue, Bet v 1.
The binding sites of eight of the 23 antibodies were also localized on myosin filaments by negative staining.
Secondary antibodies were also tested for nonspecific binding.
Anti-dsDNA antibodies were also demonstrated by ELISA using synthetic DNA duplexes as well as a filter binding assay using 3H-labeled dsEC DNA as Ag.
To determine if the hits isolated only once in the library screen were of comparable quality or inferior to those isolated multiple times, their binding to the anti-ADP3 antibodies was also analyzed using fluorescence polarization.
Anti-nuclear antibody and anti-mitochondrial antibody were also negative.
The binding time of antibody was also an important parameter, which affected the measurement of R et. Figure 5b shows the influence of antibody binding time on R et differences prior to E. coli recognition.
Euclidean distance was calculated between atom a i and a j with their coordinates a i(x i, y i, z i) and a j(x j, y j, z j) in the PDB structure data, d i j = x i - x j 2 + y i - y j 2 + z i - z j 2 Antigen residues r i whose minimum atom distance to the binding antibody is less than 4Å were also incorporated in epitope.
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