Sentence examples for binding and transition state from inspiring English sources
Two other conserved residues at the active site of XORs, GluB232 and ArgB310, are involved in substrate binding and transition state stabilization [14], [17].
The catalytic mechanism of R. capsulatus XDH was proposed to involve three amino acids at the active site: GluB730 is thought to act as an active site base in the initial step of the reaction, while GluB232 is involved in substrate binding and transition state stabilization and RB310 is involved in transition state stabilization and orientation of the substrate at the active site.
βArg-182 is strongly conserved and critical for Pi binding and transition state stabilization.
In summary, the αThr-349 residue of the conserved VISIT-DG sequence in the ATP synthase α-subunit is required for catalysis, Pi binding, and transition state stabilization.
The likely H-bond interaction between αThr-349 and βArg-182 (and αArg-376) suggests these residues act together to support Pi binding and transition state stabilization.
The results demonstrate that αThr-349 is a supplementary residue involved in phosphate binding and transition state stabilization in ATP synthase catalytic sites through its interaction with βR182.
Mutations that affect substrate carboxyl binding (R71N) and transition state stabilization (R63N) also yielded wild-type aspartoacylase protein levels and undetectable aspartoacylase activity.
In addition, GluB232 and ArgB310 of R. capsulatus XDH were shown to be involved in the binding, orientation and transition state stabilization of the substrate [14], [17].
Enzyme inhibitors of ATP-dependent enzymes such as kinases are needed to examine the binding sites and transition states involved in ATP-dependent reactions.
This work suggests that this particular system exhibits uniform binding with respect to ground state and transition state and provides insight into the challenge of re-engineering a Mg2+-dependent DNAzyme with enhanced catalytic activity.
The αT349A mutant was used to appreciate the role of the Thr-OH side chain in Pi binding and the transition state.
