We wanted to test if deletion of sdc-3 would alter chemokine binding and presentation at the endothelial surface, which may explain the reduced neutrophil recruitment in sdc-3 null mice in response to CXCL1.
The reason for this is not clear, but may simply be due to differences in the binding and presentation of epitopes between the distinct MHC class II haplotypes.
In a subgroup of three laboratories (Z5, Z6 and Z8), an allogeneic APC population (T2 or K562-A*0201 cells) was added for binding and presentation of the synthetic peptides.
In both α-GalCer and ThrCer series, the urea analogues displayed poor activity, and we were unable to obtain TCR binding and kinetics data for these two substrates, which may suggest that the additional NH functionality incorporated into the acyl chain disrupts glycolipid binding and subsequent presentation.
CD1d agonists, which lead to biased Th1/Th2 responses, have received particular attention, while α-GalCer analogues have also helped to elucidate the structural requirements for CD1d binding and subsequent presentation to iNKT cell TCRs.
MHC class I molecules, for instance, are involved in the binding and presentation of intracellular generated peptides to CD8+ T cells, whereas class II molecules present peptides from extracellular origin to CD4+ T cells.
The highly polymorphic HLA DR, DQ, and DP loci are encoded by genes in the human major histocompatibility complex (MHC), and are responsible for the binding and presentation of infection-derived peptides to CD4+ T cells, leading to adaptive immune responses to infections (Cooke and Hill, 2001).
The divergence among the pertactin alleles is proximal to the encoded RGD motif that is involved in eukaryotic cell binding and antigen presentation to B cells (21 ).
Likewise, a negative association suggests that the binding and presentation of a peptide is effective and elicits a successful T-cell cytotoxic response with destruction of leukemia cells.
Functional clustering of all differentially regulated genes in either of the two methods revealed genes involved in stress response, energy production, transcription, RNA processing, protein synthesis and degradation, growth control, adhesion, cytoskeletal organisation, Ca2+ binding and antigen presentation.
